Daily View
Thursday, September 24, 2015

UPSC-Bio4Energy Guest Lecture: Genetic engineering of lignin using a bacterial gene, Umeå, Sweden (All day)

Prof. Shinya Kajita (TUAT, Japan) will be coming to Umeå to give a seminar on his latest work strategies to modulate lignin in tree biomass on Thursday 24th September. His breakthrough strategy has been to use genes isolated from bacteria living off the lignin residues from pulp mill stream into poplar wood to modify in vivo the wood structure.

Bio4Energy researchers who would like to discuss with Prof. Kajita on Thursday 24th September should contact This email address is being protected from spambots. You need JavaScript enabled to view it. to set a meeting time.

Shinya Kajita

Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology

Title of the seminar: Genetic engineering of lignin using a bacterial gene

Lignin is one of the major components of the plant cell wall. It is an aromatic polymer with different types of chemical linkage. The most abundant linkage unit in typical native dicot lignin is the β-aryl ether (β–O–4) unit, which accounts for over 50% of all units. The benzylic α-positions of β–O–4-units are usually hydroxy-substituted. The α-keto-β–O–4 units, with carbonyl groups at the benzylic positions, are also found in natural lignins at very low concentrations. These α-keto β–O–4 units can be cleaved under alkaline and/or oxidative conditions more easily and faster than the typical β–O–4-units with benzylic hydroxyl groups. Thus, increasing the abundance of α-keto-β–O–4 units as opposed to the typical α-hydroxy-β–O–4 units in the lignin backbone can contribute to a reduction in the cost and energy required for chemical pulping and biomass pretreatment processes in cellulosic ethanol production.


Sphingobium sp. strain SYK-6 , a gram-negative bacterium, can utilize various monomeric and dimeric aromatic compounds that are intermediates in the lignin biosynthetic pathway, such as cinnamic acid, cinnamaldehyde, and β–O–4 dimers. In our previous studies, we isolated and characterized a lot of genes from the bacterium, which were involved in the degradation of these compounds. One of the genes, ligD, encodes Cα dehydrogenase, which catalyzes the first step in the cleavage of the ether bond of β–O–4 dimers. This enzyme oxidizes the alcohol group at benzyl position of the dimers and oligomers to the carbonyl group. Thus, in the present study, we introduced ligD into the plant genome and attempted to generate transgenic plants whose lignin can be easy to remove from the holocellulose fraction. Recombinant LigD , and transgenic Arabidopsis plants with ligD and their lignins have been characterized by chemical, biochemical, and genetic methods.

Reference: Tsuji et al. Plant Biotech J, 13, 821-832 (2015).

Conference: Bioenergy Networks and Biomass Potentials: Valorising European Organic Residues, Brussels, Belgium (Thu. 24 Sep, 2015 11:00 - 19:00)

 

Dear Mr/MS
On behalf of BioenNW Project Consortium, I am pleased to invite you to the:

BioenNW Final International Conference

Bioenergy Networks and Biomass Potentials: Valorising European Organic Residues 24 September 2015, EUBIA Conference Centre, Rond Point Schuman 6, B1040, Brussels, Belgium

 
Download here the Full Conference Programme!
 

The BioenNW Final International Conference will be focusing on the most promising technologies and strategies for an efficient exploitation of European biomass residues. Top level speakers, from the European Commission, leading research centres, universities and industries will be participating, for a full day on...

 

SUSTAINABLE BIOENERGY IN EUROPE

 

Follow the instruction to register: the Conference is completely free!

This event is open to a maximum of 130 participants, therefore, an advance registration is needed to secure your place in time.
Please send us your registration form before 10th of September 2015!
Looking forward to seeing you in Brussels!
Yours sincerely,
EUBIA - Partner of BioenNW Project Consortium

Categories

 
 

Search Calendar